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Abstracts

International Symposium on Infectious Salmon Anaemia

September 13–15, 2010
Thon Hotel Opera, Oslo, Norway

Virology · Epidemiology · Biosecurity

The programme includes scientific sessions and a one day workshop focusing on risk management.
Keynotes will be given by international recognized speakers.

The goal of this conference is to provide updates on ISA research, biosecurity and disease control strategies.

Venue: the symposium was held at Thon Hotel Opera, in centrum of Oslo.

Read the abstracts from the symposium

Scientific program

September 13

From 8.30 Registration

11.00 Welcome by Harald Gjein, Director of the National Veterinary Institute, Norway
11.05 Introductory speak by Vidar Ulriksen, State Secretary of the Norwegian Ministry of Fisheries and Costal Affairs
11.20 OIE and aquatic animal disease control in a historical perspective
Dr. Barry Hill, President OIE Aquatic Animal Health Commission
Centre for Environment, Fisheries and Aquaculture Science; Weymouth, UK

12.00 Section Pathology and pathogenesis
Chair Niels Jørgen Olesen
Keynote Dr Ole Bendik Dale, National Veterinary Institute, Norway

12.30 Scientific presentations 15 mins

  • Passage of infectious salmon anemia virus in rainbow trout (Oncorhynchus mykiss)
    Christel M. Olsen, Stine Bråen, Espen Rimstad
  • Infectious salmon anaemia virus (ISAV) loads in different organs in diseased and asymptomatically infected fish
    Monika Hjortaas, Elin Trettenes, Turhan Markussen

13.00 Lunch
14.30 Scientific presentations 15 mins continues

  • Infection of atlantic salmon (salmo salar) endothelial cells by Infectious salmon anaemia virus
    Maria Aamelfot, Ole Bendik Dale, Simon Weli, Erling Olaf Koppang, Knut Falk
  • ISAV infection of atlantic salmon gills
    Simon Weli, Dale Ole Bendik, Maria Aamelfot, Erling Koppang, Knut Falk
  • Experimental evidence indicating that the balance between receptor binding- and receptor destroying activity is a major factor for Infectious salmon anaemia (ISA) pathogenesis
    Knut Falk, Ole Bendik Dale
  • Depletion of CD8 alpha cells from tissues of Atlantic salmon during the early stages of infection with high or low virulent strains of infectious salmon anaemia virus (ISAV)
    Dyveke Hetland, Ole Bendik Dale, Karsten Skjødt, Charles McL. Press, Knut Falk
  • Infectious Salmon Anemia Virus (ISAV) of the HPR0 subtype is highly prevalent and causes a non-clinical transient infection in farmed Atlantic salmon (salmo salar l) in the Faroe Isla
    Debes H. Christiansen, Peter S. Østergaard, Mike Snow, Knut Falk

16.00 Coffe break

16.30 Section Diagnostics – methods and procedures
Chair Debes H. Christiansen
Keynote Dr. Mike Snow, Fisheries Research Services Marine Laboratory, Aberdeen, UK

17.00 Scientific presentations 15 mins

  • Immunohistochemistry evaluated as a diagnostic method for Infectious salmon anaemia (ISA)
    Knut Falk, Ole Bendik Dale
  • A retrospective study of ISA in farmed Atlantic salmon by IHC and RT-PCR using formalin-fixed paraffin-embedded tissue samples
    Knut Falk, Ole Bendik Dale
  • Selection of ISAV diagnostic tests based upon fitness for purpose
    Charles Caraguel, Larry Hammell, Nellie Gagné, Henrik Stryhn, Ian Dohoo

18.00 Ends, Get toghether with a bit of food (seafood buffet) and wine

September 14

9.00 Section Epidemiology
Chair Rob Raynard
Keynote Dr Lori Gustafson, National Surveillance Unit, USDA/APHIS/VS/CEAH, Fort Collins, USA

9.35 Scientific presentations 15 mins

  • The Development and Epidemiological Aspects of the Infectious Salmon Anemia (ISA) in Chile: 2007 to 2010
    Marcus Godoy
  • Epidemiology and the confinement and eradication of an outbreak of ISA in Shetland, Scotland
    Alexander Murray, Lorna Munro, Stuart Wallace, Daniel Pendrey, David Fraser, Mike Snow, Rob Raynard
  • Caligus Rogercressey as vector of ISA virus
    Siri Vike, Karin Oelckers, Henrik Duesund, Javier Gonzalez, Are Nylund
  • Survival and shedding of Infectious Salmon Anemia virus (ISAV) during decomposition of dead ISA Virus positive Atlantic salmon (Salmo salar)
    Henrik Duesund, Siri Vike, Are Nylund

10.35 Coffe Break

11.00 Scientific presentations 15 mins continues

  • Infectious salmon anaemia (ISA) in Norwegian salmon farming, a chronicle of events relating to a small scale epidemic
    Einar Karlsen, Eva Jeanette Johansen, Trude M. Lyngstad, Peder A. Jansen
  • Using molecular epidemiology to trace the spread of infectious salmon anaemia virus (ISAV) in Norwegian salmon farming
    Trude M. Lyngstad, Monika J. Hjortaas, Anja B. Kristoffersen, Turhan Markussen, Einar Karlsen, Christine M. Jonassen, Peder A. Jansen
  • Modeling the spread of infectious salmon anaemia (ISA) in marine salmon farms in Norway based on sea distances between farms and genetic distances between the ISA viruses from different outbreaks
    Peder Jansen, Magne Aldrin, Trude M. Lyngstad, Anja B. Kristoffersen, Bård Storvik, Ørnulf Borgan

11.45 Section Virus characterisation and host interactions
Chair Brit Hjeltnes
Keynote Professor Espen Rimstad, Norwegian School of Veterinary Science, Norway

12.15 Scientific presentations 15 mins

  • Infectious salmon anemia: virus-host interactions
    Jon NE Ramsell, Helena Hauge, Mehrdad Sobhkhez, Jorunn Jørgensen, Siri Mjaaland
  • Targeting of Infectious salmon anemia virus antigens to salmonid antigen presenting cells
    Helena Hauge, Jon Ramsell, Ida Hedfors, Unni Grimholt, Siri Mjaaland
  • Virulence markers in Haemagglutinin-esterase (HE) protein (Highly Polymorphic Region) and Fusion Protein (Q266-L266) in infectious salmon anaemia virus (ISAV) in Chile
    Godoy Marcos, Fred Kibenge, Valentina Gherardelli, Paola Olmos, Loreto Ovalle, Gerardo Muñoz, Molly Kibenge, Yingwei Wang, Alicia Gallardo, Lara M., Avendaño-Herrera R

13.00 Lunch

14.00 Scientific presentations 15 mins continues

  • Characterization of the protein encoded by the spliced gene product of genomic segment 7 of infectious salmon anemia virus (ISAV)
    Rimaltuhana binti Ramly, Christel Moræus Olsen, Turhan Markussen, Espen Rimstad
  • Development of a rapid and universal method for the characterization of gene segment untranslated regions from different strains of infectious salmon anaemia virus
    Mickael Fourrier, Sandra Heuser, Eann Munro, Mike Snow
  • Presence of a full length highly polymorphic region (HPR) in the ISAV haemagglutinin-esterase does not affect the primary functions of receptor binding and esterase activity
    Alastair McBeath, Mickael Fourrier, Knut Falk, Mike Snow

14.45 Section Vaccination and disease control
Chair Roar Gudding
Keynote Professor Larry Hammell, Atlantic Veterinary College, PEI, Canada

15.15 Scientific presentations 15 mins

  • Evaluation of efficacy of infectious salmon anemia virus (ISAV) plasmid vaccines compared with a conventional ISAV virin vaccine in Atlantic salmon
    Kira Salonius, Nathalie Simard, Linda Bootland
  • Clinical relevance and use of ISA vaccines in effected regions of North America, Chile, Faeroes and Norway
    Kira Salonius, Allison Mackinnon, Constantino Siderakis , Neil Robertson, Lars Speilberg
  • Clinical laboratory studies as tools in ISA vaccine development
    Frode Fridell, Edel Anne Norderhus, Claudia Maira, Inge-Tom Solbakk, Anne Aas-Eng, Kjetil Fyrand
  • Immunogenicity of an inactivated infectious salmon anemia virus vaccine in Atlantic salmon (Salmo salar)
    Astrid Lauscher, Bjørn Krossøy, Petter Frost, Søren Grove, Melanie König, Lars Austbø, Espen Rimstad
  • ISAV infection and innate immunity: does autophagy play a role?
    Tor Gjøen

16.30 Coffee/Poster session

  • Preliminary characterisation of a putative monoclonal antibody marker (10E4) for Atlantic salmon (salmo salar) endothelial cells
  • Complete Genome Sequencing and Phylogenetic Analysis of Five Chilean isolates of Infectious Salmon Anemia Virus (ISAV)
  • Vaccine cross-protection against Chilean, Norwegian and North American strains of Infectious Salmon Anaemia Virus (ISAV)
  • Analysis of the transmission pathways for infectious salmon anaemia virus (ISAV) in Norwegian salmon farming
  • Dispersal of ISA virus in Chile: An international collaboration initiative
  • Detection of Infectious Chilean Salmon Anaemia Virus Strains by Shell Vial Assay
  • Observations on the behaviour Chilean Strains of Infectious Salmon Anaemia Virus in cell culture

17.15 Ends

19.00 Dinner at Ekeberg Restaurant

September 15 - Workshop

9.00 Strategies for biosecurity and risk management in industrial aquaculture.
Dr. Hernán Rojas Olavarría, CERES Biosecurity and Quality, Chile

9.35 Summarizing strategies and experiences on ISA prevention, control and eradication in the respective countries.
15 mins Presentations from

  • Canada/USA
  • Chile
  • Faroe Islands
  • Norway
  • Scotland

11.05 Coffee Break

11.30 Classification of fish farms and surveillance of ISA from an EU perspective
Dr Niels Jørgen Olesen, VI Danmark
12.00 ISA control/eradication strategies from an industrial perspective
Siri Vike, Mainstream
12.30 General discussion
13.00 Lunch
14.00 Challenges associated with different prevention/control/eradication strategies.

  • Surveillance strategies and screening
  • Transmission of ISAV- importance of HPR0
  • Eradication of ISA – eradication of ISAV?
  • Disease control

Panel and plenary discussion on ISA prevention, control, and eradication
Dr. Hernán Rojas Olavaria
15.45 Conclusion/Summary by Brit Hjeltnes, Head of Department for Fish and Shellfish Health, National Veterinary Institute
16.00 Ends, Harald Gjein, Director of the National Veterinary Institute, Norway

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