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Cytosol protein regulation in H295R steroidogenesis model induced by the zearalenone metabolites, alpha- and beta-zearalenol

Busk OL, Frizzell C, Verhaegen S, Uhlig S, Connolly L, Ropstad E, Sorlie M.  Cytosol protein regulation in H295R steroidogenesis model induced by the zearalenone metabolites, alpha- and beta-zearalenol. TOXICON 2012; 59 (1):17-24

alpha- and beta-zearalenol (alpha-ZOL and beta-ZOL, respectively) are metabolites of the mycotoxin zearalenone (ZEN). All three individual mycotoxins have shown to be biological active i.e. being estrogenic and able to stimulate cellular proliferation albeit at different strengths. In this work, cytosol protein expression was determined by using stable-isotope labelling by amino acids in cell culture (SILAC) upon exposure of alpha-ZOL and beta-ZOL to the steroido-genesis cell model H295R. A total of 14 and 5 individual proteins were found to be significantly regulated by alpha-ZOL and beta-ZOL, respectively. Interestingly, there were no common protein regulations by the metabolites or the parent mycotoxin ZEN. Furthermore, the regulated proteins were assigned to networks and groups of actions that also differed from one another suggesting that the three individual mycotoxins may have unique biological activities. (C) 2011 Elsevier Ltd. All rights reserved.

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